JS Nie CM
W
The term is somewhat misleading in that 1 µg of plasmid is rarely actually transformed. OL K
(1990)
JH S
Lyon SP H Huang You can also search for this author in 2013 Nov;67(5):550-6. doi: 10.1007/s00284-013-0400-z. Certain strains of bacteria are more easily transformed than others. Nester
G Factors affecting transformation efficiency. S
Jeon
Vijaykanth
• Depending on the nature of DNA plasmids and how its stored • Depending on the DNA plasmids concentrations • Depending on DNA's optimal concentration for transformation
Rathore K Van Montagu doi: 10.1002/mbo3.1043. G
Wasmann
G Van den Eeckhout F M MR Individual cells are capable of taking up many DNA molecules, but the presence of multiple plasmids does not significantly affect the occurrence of successful transformation events. Swain
Experiment of natural transformation later proves the existence of the phenomenon of natural transformation and it's also related to factors such as calcium chloride concentration, plasmid concentration and others.
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Beijing 2009 Jul;83(5):791-8. doi: 10.1007/s00253-009-2042-4.
JW VG MooreAn evaluation of factors affecting the efficiency of Agrobacterium mediated transformation of Citrus paradisi (Macf.) (1962)
On the whole, the efficiency of Ca(2+) was found to be the optimum bivalent ion, following by Sr(2+),Mn(2+),Mg(2+),Ba(2+).
Montagu M
Nie
Transformation efficiency can be measured in transformants or colony forming unit (cfu) per μg DNA used. Kumria Further dilution may be used for high efficiency transformation. S
Conner
Weeks Umbeck Tanksley
MD
Vergauwe
E Smith
J
(1987) Wan K Individual cells are capable of taking up many DNA molecules, and that the presence …